Improved serum-free culture conditions for spleen-derived murine fibrocytes
نویسندگان
چکیده
منابع مشابه
DIFFERENTIATION OF MONOCYTE DERIVED DENDRITIC CELLS IN SERUM FREE CONDITIONS
Human peripheral blood monocytes (HPBM) were cultured in the absence of human serum and were converted into a state exhibiting a high accessory function expressed by their ability of supporting lymphocyte proliferation. After a prolonged culture in serum free media the monocyte derived cells were highly viable, increased in size and developed veils and dendritiform elongatio'l1s. Paralleli...
متن کامل[Mesenchymal cells and fibrocytes in peripheral blood. Culture conditions].
OBJECTIVES The fibrocytes, the mesenchymal cells and the colony forming units are included in the concept fibroblast-like, peripheral-blood circulating stem cells. The aim of this work was to establish the culture conditions. MATERIAL AND METHOD Peripheral blood mononuclear cells were isolated from 25 samples collected from the cephalic vein. These were cultured in McCoy's 5A enriched with 20...
متن کاملInterleukin-6 enhances murine megakaryocytopoiesis in serum-free culture.
We investigated the effect of interleukin-6 (IL-6) on murine megakaryocytopoiesis in a serum-free culture system. The addition of IL-6 to a culture containing interleukin-3 (IL-3) resulted in a significant increase in the number of megakaryocyte colonies by bone marrow cells of normal mice. The megakaryocytic progenitors that survive exposure to 5-fluorouracil (5-FU) exhibited a more significan...
متن کاملdifferentiation of monocyte derived dendritic cells in serum free conditions
human peripheral blood monocytes (hpbm) were cultured in the absence of human serum and were converted into a state exhibiting a high accessory function expressed by their ability of supporting lymphocyte proliferation. after a prolonged culture in serum free media the monocyte derived cells were highly viable, increased in size and developed veils and dendritiform elongatio'l1s. paralleli...
متن کاملImproved serum- and feeder-free culture of mouse spermatogonial stem cells Running title: Serum-free culture of spermatogonia
Spermatogonial stem cells (SSCs) undergo self-renewal division, which can be recapitulated in vitro. Attempts to establish serum-free culture conditions for SSCs have met with limited success. Although we previously reported that SSCs can be cultured without serum on laminin-coated plates, the growth rate and SSC concentration were relatively low, which made it inefficient for culturing large n...
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ژورنال
عنوان ژورنال: Journal of Immunological Methods
سال: 2010
ISSN: 0022-1759
DOI: 10.1016/j.jim.2010.09.025